Melanoma-associated genes, MXI1, FN1, and NME1, are hypoxia responsive in murine and human melanoma cells.

摘要: Hypoxia can influence aggressiveness of melanoma by inducing specific gene expression profiles. In our previous microarray study, we identified more than 430 hypoxia-responsive genes in the B16-F10 murine cell line vitro. Of identified, seven genes: galectin 3 (Lgals3), adhesion molecule (Mcam), fibronectin 1 (Fn1), signal transducer and activator transcription (Stat3), microphthalmia-associated factor (Mitf), max interacting protein (Max1), non-metastatic cells 1, (NM23A) expressed (Nme1) are known to be associated with melanoma, but have not yet been reported as being regulated hypoxia human cells. this investigated whether these is modulated microdissected areas experimental tumors vivo, well commercially available lines (WM35, WM1552C, WM793B, WM278, 1205Lu, 451Lu) exposed hypoxic conditions Our analysis revealed significant agreement between in-vitro in-vivo results showing that all except Mitf were oxygen-deprived tumor regions (P<0.05). contrast, three (NME1, MXI1 FN1) proved both mouse link genes, for first time, microenvironment imply widely used model could a convenient research tool further investigation their role development course malignancy.