Isolation and Characterization of Ribonucleic Acid from Cerebral Cortex of Rat
作者： H.R. Mahler , Walter J. Moore , Ralph J. Thompson
DOI: 10.1016/S0021-9258(18)96771-2
关键词: Sucrose gradient centrifugation 、 RNA 、 Ribosomal RNA 、 Ultracentrifuge 、 Biochemistry 、 Ribosome 、 Rat brain 、 Albumin 、 Biology 、 Chromatography 、 Cerebral cortex 、 Cell biology 、 Molecular biology
摘要: Abstract Ribonucleic acid isolated from cerebral cortex of mature male and female rats was fractionated by chromatography on methylated albumin Kieselguhr columns or sucrose gradient centrifugation. Three principal fractions were obtained with analytical ultracentrifuge s20,w0 values 4, 17, 28. By comparison ribonucleic prepared purified rat brain ribosomes, the 17 S 28 shown to be predominantly ribosomal acid. Differences in base analyses between three small but significant. Compositions all markedly complementary G ≈ C A U.
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