Further studies on the heterogeneity of lens ribonucleic acid
作者： G.J. Köteles , F. Antoni , A. Takáts
DOI: 10.1016/S0014-4835(68)80068-5
关键词: Similar distribution 、 Biochemistry 、 Sucrose 、 Biology 、 Phenol 、 Lens (optics) 、 Albumin 、 RNA 、 Column chromatography 、 Ultracentrifuge
摘要: Ribonucleic acid (RNA) from separated parts of cattle lenses, i.e. epithelial cells, cortical and nuclear fibres was prepared by phenol treatment. The heterogeneity ribonucleic studied sucrose density-gradient ultracentrifugation methylated albumin kieselguhr column chromatography. Due to the differentiation cells lens fibres, marked decrease in relative amounts high molecular weight RNA an increase low were observed both methods. Similar distribution shown when chick lenses during ontogenesis, starting embryonic stage, studied. method chromatography proved be more advantageous features RNA, present large quantity lens, are
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sci-hub.se 参考文章(23)
Sidney Lerman, Metabolic Pathways in Experimental Sugar and Radiation Cataract Physiological Reviews. ,vol. 45, pp. 98- 122 ,(1965) , 10.1152/PHYSREV.1965.45.1.98
H.R. Mahler, Walter J. Moore, Ralph J. Thompson, Isolation and Characterization of Ribonucleic Acid from Cerebral Cortex of Rat Journal of Biological Chemistry. ,vol. 241, pp. 1283- 1289 ,(1966) , 10.1016/S0021-9258(18)96771-2
Abraham Spector, Dorothy Travis, Protein Synthesis and Polyribosomes in the Calf Lens Journal of Biological Chemistry. ,vol. 241, pp. 1290- 1295 ,(1966) , 10.1016/S0021-9258(18)96772-4
R. B. Scott, E. Bell, Messenger RNA Utilization during Development of Chick Embryo Lens Science. ,vol. 147, pp. 405- 407 ,(1965) , 10.1126/SCIENCE.147.3656.405
H. Bloemendal, J. Schoenmakers, A. Zweers, R. Matze, E.L. Benedetti, Polyribosomes from calf-lens epithelium Biochimica et Biophysica Acta. ,vol. 123, pp. 217- 220 ,(1966) , 10.1016/0005-2787(66)90179-1
Donald G. Comb, Nilima Sarkar, The binding of 5 s ribosomal ribonucleic acid to ribosomal subunits Journal of Molecular Biology. ,vol. 25, pp. 317- 330 ,(1967) , 10.1016/0022-2836(67)90144-1
Joseph D. Mandell, A.D. Hershey, A fractionating column for analysis of nucleic acids Analytical Biochemistry. ,vol. 1, pp. 66- 77 ,(1960) , 10.1016/0003-2697(60)90020-8
F. Antoni, G.J. Köteles, Magda Radnót, Studies on the nucleic acid content and the heterogeneity of ribonucleic acid in the rabbit lens Experimental Eye Research. ,vol. 2, pp. 65- 70 ,(1963) , 10.1016/S0014-4835(63)80026-3
N. Virmaux, P. Mandel, P.F. Urban, Evidence of two soluble RNA types in eye lens Biochemical and Biophysical Research Communications. ,vol. 16, pp. 308- 313 ,(1964) , 10.1016/0006-291X(64)90031-2
R. J. Britten, R. B. Roberts, High-Resolution Density Gradient Sedimentation Analysis Science. ,vol. 131, pp. 32- 33 ,(1960) , 10.1126/SCIENCE.131.3392.32