Far-Red Fluorescent Protein Excitable with Red Lasers for Flow Cytometry and Superresolution STED Nanoscopy

摘要: Far-red fluorescent proteins are required for deep-tissue and whole-animal imaging multicolor labeling in the red wavelength range, as well probes excitable with standard lasers flow cytometry fluorescence microscopy. Rapidly evolving superresolution microscopy based on stimulated emission depletion approach also demands genetically encoded monomeric to tag intracellular at molecular level. Based mKate variant, we have developed a far-red TagRFP657 protein excitation/emission maxima 611/657 nm. has several advantages over existing including higher photostability, better pH stability, lower residual green fluorescence, greater efficiency of excitation lasers. The red-shifted spectra, compared other proteins, allows utilizing simultaneously orange or near-red proteins. is shown be an efficient using commercially available microscope.