Loss of heterozygosity in cervical carcinoma: subchromosomal localization of a putative tumor-suppressor gene to chromosome 11q22-q24

摘要: Infection of cervical epithelial cells with so-called "aggressive" subtypes human papilloma virus (HPV) appears to be an important factor in the etiology carcinoma. However, mounting evidence suggests that additional genetic changes are required for progression invasive Functional studies have shown chromosome 11 contains a gene or genes capable suppressing tumorigenicity cell lines derived from different histopathological types carcinoma, suggesting aberration this gene(s) may represent at least one tumorigenic progression. To identify likely chromosomal position gene(s), we carried out systematic analysis primary tumors 32 patients Sixteen highly polymorphic markers, 10 which were based on simple sequence repeats typed by PCR, used compare matched DNA samples noninvolved tissue and portions tumor enriched neoplastic cryostat-sectioning technique. Of examined, 14 (44%) demonstrated clonal alterations resulting loss heterozygosity more markers. Seven specific long arm, overlap between these other allelic deletions suppressor relevant carcinoma maps 11q22-q24.