Site-directed psoralen crosslinking of DNA

摘要: Abstract A technique has been developed for placing site-specific crosslinks into DNA by using a psoralen derivative containing sulfhydryl group. Plasmid pBR322 was nicked at the unique BamHI restriction site, and mercurated pyrimidines were introduced nick-translation. Then specifically directed to this site in dark through Hg-S linkage prior irradiation form crosslink. The location of interstrand cross-links examined electron microscopy Pst I-cut molecules found be or near site.