Process for labeling nucleic acids using psoralen derivatives

摘要: A labeling reagent of the formula: [A][B]L is prepared where an alkylating intercalation moiety, B a divalent organic spacer arm moiety with straight chain at least two carbon atoms, and L monovalent label capable producing detectable signal, e.g., signal by spectroscopic, photochemical, chemical, immunochemical or biochemical means. Preferably 4'-methylene-substituted psoralen most preferably 4,5',8-trimethylpsoralen moiety. This may be used to nucleic acids, DNA, intercalating into partially double-stranded acid form complex activating cause covalent bonding between acid. Preferably, labeled hybridization probe for detecting sequences hybridizing hydridizing region Also non-radioactive. also in chromosome banding specific regions chromosomes thereby differentiate them.