DERIVATIVES OF PYRAZOLO[3,4-d]PYRIMIDINE

摘要: This invention is directed to novel 3,4-disubstituted and 3,4,6-trisubstituted pyrazolo[3,4-d]pyrimidines the use of these compounds in preparation oligonucleotides. The also nucleosides mono- oligonucleotides comprising at least one pyrazolopyrimidines, resulting for diagnostic purposes. More particularly, pyrazolopyrimidines present are formula (I), wherein R1 hydrogen, or a sugar moiety optionally substituted its 3' 5' position with monophosphate, diphosphate, triphosphate, reactive group suitable nucleotide bond formation; provided that when R3 then cannot be hydrogen; hydrogen -W-(X)n-A; each W X independently chemical linker arm; A an intercalator, electrophilic crosslinker reporter group; R4 R6 H, OH, SR, NH2, NH(CH2)tNH2; R H C1-6alkyl; n zero one; t twelve. oligo- polynucleotides useful identification, isolation, localization and/or detection complementary nucleic acid sequences interest cell-free cellular systems. Therefore, further provides method identifying target sequences, which comprises utilizing polynucleotide probe labeled pyrazolo[3,4-d]pyrimidine invention.