Cells with clonal light chains are present in peripheral blood at diagnosis and in apheretic stem cell harvests of primary amyloidosis.

摘要: In primary systemic amyloidosis, small numbers of bone marrow plasma cells secrete monoclonal light chains that form extracellular fibrils (amyloid) in various organs. Evidence limited to a few cases suggests rare clonal elements can also be found the peripheral blood (PB), and this may relevant PB stem cell autotransplantation. Since up 40% amyloid clones do not synthesize heavy chains, order detect tumor with high specificity sensitivity we developed seminested allele-specific oligonucleotide polymerase chain reaction for chains. Clone-related sequences were detected DNA and/or cDNA from eight 10 patients at diagnosis apheretic collections three four undergoing progenitor there are experimental data suggesting circulating involved growth amyloidogenic clone chemoresistant, these findings use leukapheresis purging strategies autotransplantation amyloidosis.