Use of coisogenic host blastocysts for efficient establishment of germline chimeras with C57BL/6J ES cell lines.
作者： K. Schuster-Gossler , A.W. Lee , C.P. Lerner , H.J. Parker , V.W. Dyer
DOI: 10.2144/01315ST01
关键词: Embryonic stem cell 、 Blastocyst 、 Germline 、 Cell biology 、 Embryogenesis 、 Genetics 、 Stem cell 、 Biology 、 Cell culture 、 Gene targeting 、 Chimera (genetics)
摘要: Gene targeting in embryonic stem (ES) cells allows the production of mice with specified genetic mutations. Currently, germline-competent ES cell lines are available from only a limited number mouse strains, and inappropriate cell/host blastocyst combinations often restrict efficient gene-targeted mice. Here, we describe derivation C57BL/6J (B6) compare effectiveness two host donors, FVB/NJ (FVB) coisogenic strain C57BL/6-Tyr(c)-2J (c2J), for germline chimeras. We found that when B6 were injected into c2J blastocysts, high rate coat-color chimerism was detected, transmission could be obtained few injections. In all but one case, highly chimeric transmitted to 100% their offspring. The injection FVB blastocysts produced some However; proportion low, many more injections required generate chimeras capable transmission. Our data support use albino strain, c2J, generation using cells.
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