Detection of aerosolized bacterial spores (Bacillus atrophaeus) using free-flying honey bees (Hymenoptera: Apidae) as collectors

作者: Bruce Lighthart , Kevin R. S. Prier , Jerry J. Bromenshenk

DOI: 10.1007/S10453-004-1182-3

关键词: SporeHorticultureBacillus atrophaeusHoney beeEndosporeHymenopteraBotanyApidaeBiologyAerosolizationHoney Bees

摘要: An aerosol cloud of Bacillus atrophaeus (previously B. subtilis variety niger) spores, an anthrax surrogate, was created in a large 0.4 ha (1 ac), bee-containing, open-mesh tent. Bees from uncontaminated hive flying through the adsorbed spores statistically significant quantities. After removal contaminated and introduction another hive, bees again were monitored for next few days spores. accumulated on bees’ bodies following their exposure to residual contamination The spore loads quickly returned background levels after hives removed tent area. It may therefore be practical use honey bee colonies monitor foraging areas disease-causing

参考文章(14)
E. Gattavecchia, M. H. Pham-Delègue, S. Girotti, S. Ghini, G. Celli, C. Porrini, A. G. Sabatini, J. Devillers, Use of honey bees as bioindicators of environmental pollution in Italy. Honey bees: estimating the environmental impact of chemicals. pp. 186- 247 ,(2002)
J. Bromenshenk, B. Lighthart, K. Prier, G.M. Loper, Bees scavenge airborne bacteria Microbial Ecology. ,vol. 39, pp. 314- 321 ,(2000) , 10.1007/S002480000014
E. L. Ghisalberti, Propolis: A Review Bee World. ,vol. 60, pp. 59- 84 ,(1979) , 10.1080/0005772X.1979.11097738
Yongyi Tong, Diurnal distribution of total and culturable atmospheric bacteria at a rural site Aerosol Science and Technology. ,vol. 30, pp. 246- 254 ,(1999) , 10.1080/027868299304822
D Fritze, R Pukall, Reclassification of bioindicator strains Bacillus subtilis DSM 675 and Bacillus subtilis DSM 2277 as Bacillus atrophaeus International Journal of Systematic and Evolutionary Microbiology. ,vol. 51, pp. 35- 37 ,(2001) , 10.1099/00207713-51-1-35
E. H. Erickson, Surface electric potentials on worker honeybees leaving and entering the hive. Journal of Apicultural Research. ,vol. 14, pp. 141- 147 ,(1975) , 10.1080/00218839.1975.11099818