Interaction of Leuconostoc mesenteroides glucose-6-phosphate dehydrogenase with pyridoxal 5'-diphospho-5'-adenosine. Affinity labeling of Lys-21 and Lys-343.
作者： J R LaDine , D Carlow , W T Lee , R L Cross , T G Flynn
DOI: 10.1016/S0021-9258(19)67631-3
关键词: Biochemistry 、 Pyridoxal 、 Affinity label 、 Dehydrogenase 、 Leuconostoc mesenteroides 、 Enzyme 、 Affinity labeling 、 Chemistry 、 NAD+ kinase 、 Pyridoxal phosphate
摘要: Pyridoxal 5'-diphospho-5'-adenosine (PLP-AMP) inhibits glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides competitively with respect to glucose 6-phosphate and noncompetitively NAD+ or NADP+, Ki = 40 microM in the NADP-linked 34 NAD-linked reaction. Incubation of [3H]PLP-AMP followed by borohydride reduction shows that incorporation 0.85 mol PLP-AMP per enzyme subunit is required for complete inactivation. Both protect against this covalent modification. The proteolysis modified isolation sequencing labeled peptides revealed Lys-21 Lys-343 are sites interaction both lysyl residues 5'-phosphate (PLP) also modifies probably Lys-343. part a highly conserved region present all dehydrogenases have been sequenced. corresponds an arginyl residue other less homologous those enzymes. PLP believed interact L. at binding site. Simultaneous induces conformational changes (Kurlandsky, S. B., Hilburger, A. C., Levy, H. R. (1988) Arch. Biochem. Biophys. 264, 93-102) postulated interfere Schiff's-base formation PLP-AMP. One covalently may be located regions undergoing NAD(+)-induced changes.
sci-hub.st 参考文章(33)
Robert G. Kemp, Irwin A. Rose, COUPLING OF REDUCED PYRIDINE NUCLEOTIDE IN LEUCONOSTOC MESENTEROIDES. Journal of Biological Chemistry. ,vol. 239, pp. 2998- 3006 ,(1964) , 10.1016/S0021-9258(18)93844-5
Laura CAMARDELLA, Carla CARUSO, Bruno RUTIGLIANO, Mario ROMANO, Guido PRISCO, Fiorella DESCALZI-CANCEDDA, Human erythrocyte glucose-6-phosphate dehydrogenase. Identification of a reactive lysyl residue labelled with pyridoxal 5'-phosphate. FEBS Journal. ,vol. 171, pp. 485- 489 ,(1988) , 10.1111/J.1432-1033.1988.TB13815.X
Stig Johansson, Sven Lindstedt, Hans-Göran Tiselius, Metabolic Interconversions of Different Forms of Vitamin B6 Journal of Biological Chemistry. ,vol. 249, pp. 6040- 6046 ,(1974) , 10.1016/S0021-9258(19)42215-1
J K Tamura, J R LaDine, R L Cross, The adenine nucleotide binding site on yeast hexokinase PII. Affinity labeling of Lys-111 by pyridoxal 5‘-diphospho-5‘-adenosine. Journal of Biological Chemistry. ,vol. 263, pp. 7907- 7912 ,(1988) , 10.1016/S0021-9258(18)68583-7
N A Morjana, C Lyons, T G Flynn, Aldose reductase from human psoas muscle. Affinity labeling of an active site lysine by pyridoxal 5'-phosphate and pyridoxal 5'-diphospho-5'-adenosine. Journal of Biological Chemistry. ,vol. 264, pp. 2912- 2919 ,(1989) , 10.1016/S0021-9258(19)81699-X
R D Rakov, J K Tamura, R L Cross, Affinity labeling of nucleotide-binding sites on kinases and dehydrogenases by pyridoxal 5'-diphospho-5'-adenosine. Journal of Biological Chemistry. ,vol. 261, pp. 4126- 4133 ,(1986) , 10.1016/S0021-9258(17)35634-X
R. D. DeMoss, I. C. Gunsalus, R. C. Bard, A GLUCOSE-6-PHOSPHATE DEHYDROGENASE IN LEUCONOSTOC MESENTEROIDES Journal of Bacteriology. ,vol. 66, pp. 10- 16 ,(1953) , 10.1128/JB.66.1.10-16.1953
H S Penefsky, Reversible binding of Pi by beef heart mitochondrial adenosine triphosphatase. Journal of Biological Chemistry. ,vol. 252, pp. 2891- 2899 ,(1977) , 10.1016/S0021-9258(17)40446-7
Jonathan JEFFERY, Jane BARROS-SODERLING, Lynda MURRAY, Irene WOOD, Robert HANSEN, Bela SZEPESI, Hans JORNVALL, Glucose-6-phosphate dehydrogenase. Characteristics revealed by the rat liver enzyme structure. FEBS Journal. ,vol. 186, pp. 551- 554 ,(1989) , 10.1111/J.1432-1033.1989.TB15242.X
Bahram Haghighi, T. Geoffrey Flynn, H. Richard Levy, Glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides. Isolation and sequence of a peptide containing an essential lysine Biochemistry. ,vol. 21, pp. 6415- 6420 ,(1982) , 10.1021/BI00268A015