Synergistic effects of bovine respiratory syncytial virus and non-cytopathic bovine viral diarrhea virus infection on selected bovine alveolar macrophage functions.

摘要: The effect of bovine respiratory syncytial virus (BRSV) and non-cytopathic viral diarrhea (ncpBVDV) infection on selected alveolar macrophage (AM) functions was investigated. Alveolar macrophages were harvested from 2- to 6-month-old calves seronegative for BRSV BVDV inoculated with approximately 1 median cell culture infective dose per AM. Control, infected, ncpBVDV-infected BRSV-ncpBVDV coinfected AM cultures evaluated Fc receptor expression, phagosome-lysosome fusion, superoxide anion (O2-) production, chemotactic activity Days 1, 3, 5, 7 post-infection. Both single combined infections significantly depressed secretion factors a more significant synergistic depression seen in coinfection. Production O2- by not decreased either or ncpBVDV infection, but coinfection BRSV-ncpBVDV. present study confirms previous reports effects indicate that affects vitro. Coinfection produced functions.