Characterization of photodynamic actions of rose bengal on cultured cells.

摘要: Purpose. The authors have previously reported successful photodynamic occlusion of corneal blood vessels using intravenous rose bengal and argon green laser irradiation. To explore the action mechanism this novel technique photothrombosis, they examined effect on cultured fibroblasts, smooth muscle cells, vascular endothelium—the cellular components vessels. Methods. Five types cells were exposed to different concentrations irradiated cell areas analyzed by fluorescence microscopy fluorometry. Various potential quenchers proteins tested for their modulation action. Results. Upon irradiation with 16 W/cm 2 light in conjunction extending above 1 X 10~ 4 M, all showed a dose-dependent photobiologic characterized constriction detachment laser-irradiated region from rest monolayer. In addition, there was dye photobleaching development blue shift excitation emission maxima areas. Binding intracellular demonstrated fluorometry showing red maximum compared solution. This binding prerequisite expression described effect, because polymer-conjugated (Sensitox II) failed reproduce it. addition native or heat-inactivated bovine serum albumin catalase decreased also owing binding, as indicated G-75 Sephadex gel nitration chromatography. Conclusion. These results indicate that specific monolayer contraction, which simulates vasoconstriction seen during photothrombosis under irradiation, appears be caused photochemical interaction bound components. Invest Ophthalmol Vis Sci. 1994;35:3295-3307.