Immunocapture of CD133-positive cells from human cancer cell lines by using monodisperse magnetic poly(glycidyl methacrylate) microspheres containing amino groups.
作者： Wei-Chih Kuan , Daniel Horák , Zdeněk Plichta , Wen-Chien Lee
DOI: 10.1016/J.MSEC.2013.09.009
关键词: Cancer stem cell 、 Glycidyl methacrylate 、 Swelling 、 Cell culture 、 Dispersity 、 Conjugated system 、 Nuclear chemistry 、 Materials science 、 Polymerization 、 Antibody 、 Polymer chemistry
摘要: Magnetic poly (glycidyl methacrylate)-based macroporous microspheres with an average particle size of 4.2 μm were prepared using a modified multi-step swelling polymerization method and by introducing amino functionality on their surfaces. Antibody molecules were oxidized on their carbohydrate moieties and bound to the amino-containing magnetic microspheres via a site-directed procedure. CD133-positive cells could be effectively captured from human cancer cell lines (HepG2, HCT116, MCF7, and IMR-32) by using …
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