Protection of B cells against the effect of alloxan.
作者： Mohamed S. Abdel-Rahman , Fatma I. Elrakhawy , Fakhry A. Iskander
DOI: 10.1016/0378-4274(92)90007-7
关键词: Superoxide dismutase 、 Internal medicine 、 Degranulation 、 Biology 、 Insulin 、 Mitochondrion 、 Alloxan 、 Pancreas 、 Islet 、 Endocrinology 、 Toxicity
摘要: Abstract Alloxan induces diabetes in laboratory animals through the destruction of endocrine pancreatic B cells. The mechanism alloxan toxicity is still obscure. This study was conducted to investigate effects Superoxide dismutase (SOD) or reduced nicotinamide adenine dinucleotide (NADPH) treatment on cells isolated rat islets prior treatment. Islets were treated with SOD (1000 U) 0.1 mM NADPH for 10 min followed by (0.18 mg) 5 min. Insulin secretion studied samples incubated 60 media supplemented glucose (1.8 mg ml ). Morphological examinations fixed after significantly protected from cytotoxic effect alloxan. Although decreased insulin 35% control, increased this level 73% control values. did not provide any protection islets. and different that alone. changes observed alone presence NADPH. exhibited multiple cellular necrosis, marked degranulation extensive vesiculation endoplasmic reticulum Golgi complex. Mitochondrial enlargement disrupted cristae mitochondrial ruptures prominent. However, similar except enlarged mitochondria. reinforces free radical hypothesis toxicity. markedly mitochondria one targets which destroyed
sci-hub.se 参考文章(21)
Malaisse Wj, Ravazzola M, Malaisse-Lagae F, Amherdt M, Renold Ae, Orci L, Perrelet A, Islet cell membrane alteration by diabetogenic drugs. Laboratory Investigation. ,vol. 34, pp. 451- 454 ,(1976)
L. G. Thaete, R. K. Crouch, M. G. Buse, S. S. Spicer, The protective role of copper-zinc superoxide dismutase against alloxan-induced diabetes: morphological aspects. Diabetologia. ,vol. 28, pp. 677- 682 ,(1985) , 10.1007/BF00291975
A. LAZAROW, J. W. PATTERSON, S. LEVEY, The Mechanism of Cysteine and Glutathione Protection Against Alloxan Diabetes. Science. ,vol. 108, pp. 308- 309 ,(1948) , 10.1126/SCIENCE.108.2803.308
LENNART BOQUIST, LENNART NELSON, RONNY LORENTZON, Uptake of labeled alloxan in mouse organs and mitochondria in vivo and in vitro. Endocrinology. ,vol. 113, pp. 943- 948 ,(1983) , 10.1210/ENDO-113-3-943
Richard E. Heikkila, Bradley Winston, Gerald Cohen, Herbert Barden, Alloxan-induced diabetes-evidence for hydroxyl radical as a cytotoxic intermediate. Biochemical Pharmacology. ,vol. 25, pp. 1085- 1092 ,(1976) , 10.1016/0006-2952(76)90502-5
S.J. Cooperstein, Dudley Watkins, Effect of alloxan on islet tissue permeability: protection and reversal by NADPH. Biochemical and Biophysical Research Communications. ,vol. 79, pp. 756- 762 ,(1977) , 10.1016/0006-291X(77)91176-7
J. C. Henquin, P. Malvaux, A. E. Lambert, Alloxan-induced alteration of insulin release, rubidium efflux and glucose metabolism in rat islets stimulated by various secretagogues Diabetologia. ,vol. 16, pp. 253- 260 ,(1979) , 10.1007/BF01221952
Michael L. McDaniel, Cathy E. Roth, Cheryl A. Bry, C. Joan Fink, Jean A. Swanson, Paul E. Lacy, Effect of ethanol and n-butanol on alloxan inhibition of glucose-induced insulin release in isolated pancreatic islets Biochemical Pharmacology. ,vol. 27, pp. 1749- 1751 ,(1978) , 10.1016/0006-2952(78)90551-8
Kjell Grankvist, Stefan Marklund, Inge-Bert Täljedal, Superoxide dismutase is a prophylactic against alloxan diabetes. Nature. ,vol. 294, pp. 158- 160 ,(1981) , 10.1038/294158A0
L.A.H. Borg, Effects of alloxan on the islets of Langerhans inhibition of leucine metabolism and insulin secretion Biochimica et Biophysica Acta. ,vol. 677, pp. 257- 262 ,(1981) , 10.1016/0304-4165(81)90093-3