Whole tissue cervical mapping of HPV infection: Molecular evidence for focal latent HPV infection in humans.
作者： Anne Hammer , Maurits NC de Koning , Jan Blaakaer , Torben Steiniche , John Doorbar
DOI: 10.1016/J.PVR.2019.02.004
关键词: In patient 、 HPV infection 、 Molecular evidence 、 Oncology 、 Hysterectomy 、 Latency (engineering) 、 Virus latency 、 Internal medicine 、 Medicine 、 Cervix 、 Stain
摘要: Abstract In this study, we aimed to provide molecular evidence of HPV latency in humans and discuss potential challenges conducting studies on latency. We analyzed the entire cervix two women who underwent hysterectomy unrelated cervical abnormality. The cervices were sectioned into 242 186 sets respectively, each set was tested separately for using SPF10-PCR-DEIA-LiPA25 system. To identify whether there any transforming or productive infection, used biomarkers E4 P16INK4a stain slides immediately adjacent HPV-positive sections. detected both cervices. patient 1, 1/242 positive HPV31. 2, 13/186 HPV18 1/186 HPV53. infection very focal patients, no sign a as evaluated by markers P16INK4a. Had only one from block, probability detecting would have been 32.3% 2%, respectively.Our findings support idea that may be able establish human cervix; however, risk associated with latent remains unclear.
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