The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells

摘要: Adenosine modulates a wide variety of biological processes via adenosine receptors. In the exocrine pancreas, regulates transepithelial anion secretion in duct cells and is considered to play role acini-to-duct signaling. To identify functional receptors Cl− channels important for secretion, we herein performed experiments on Capan-1, human pancreatic cell line, using open-circuit Ussing chamber gramicidin-perforated patch-clamp techniques. The luminal addition increased negative potential difference (V te) Capan-1 monolayers with half-maximal effective concentration value approximately 10 μM, which corresponded obtained whole-cell currents single cells. effects V te, an equivalent short-circuit current (I sc), were inhibited by CFTRinh-172, cystic fibrosis transmembrane conductance regulator (CFTR) channel inhibitor. A2B receptor agonist, BAY 60-6583, I sc through CFTR channels, whereas A2A CGS 21680, had negligible effects. antagonist, PSB 603, response adenosine. Immunohistochemical analysis showed that colocalized Ezrin membranes rat ducts. elicited guinea pig These results demonstrate activating present study endorses purinergic signaling regulation secretion.