Visualization of ATP Release in Pancreatic Acini in Response to Cholinergic Stimulus: USE OF FLUORESCENT PROBES AND CONFOCAL MICROSCOPY *

摘要: Abstract The energy providing substrate ATP can be released from various cells and act extracellularly to regulate the same or neighboring cells. However, pathway for release eliciting physiological stimulus are unclear. Recently, we showed that activates P2X P2Y purinergic receptors on pancreatic ducts. Thus, it was relevant ask whether upstream acini could source of releasable what might be. We used freshly prepared rat applied conventional luminescence measurements luciferin/luciferase reaction. As a new application this reaction in confocal microscopy, monitored luciferin fluorescence as sign by single acini. In addition quinacrine mark stores, which were similar those marked with fluorescent ATP, 2′-(or-3′)-O-(N-methylanthraniloyl) adenosine 5′-triphosphate, but only partially overlapping acridine orange LysoTracker Red. functional studies show native response stimuli most importantly cholinergic stimulation, very likely epithelium. close vicinity detect about 9 μm after stimulation. is poised paracrine mediator between