Cellular localization and antiproliferative effect of peptides discovered from a functional screen of a retrovirally delivered random peptide library.

摘要: We have generated a random peptide library fused to GFP in retroviral vector system and used this screen for peptides inhibiting tumor cell growth. Four unique sequences were isolated that exhibited antiproliferative effects specifically localized the plasma membrane cytoplasmic granular compartments. Mutational analysis revealed critical residues each sequence demonstrated correlation between subcellular localization activity. Synthetic analogs of with poly-lysine internalization sequences, but not loss-of-function mutant peptides, competed parent GFP-fused peptides. The synthetic dose-dependent cells, while had no effect. Our screening approach using retrovirally expressed intracellular enables identification specific biological function potential as therapeutics.