Inhibition of Cell Proliferation by the PCNA-Binding Region of p21 Expressed as a GFP Miniprotein

摘要: Abstract p21 (WAF1/Cip1) is the only member of CIP/KIP family which has a well-characterized PCNA-binding domain. known to have an important function in coordination cellular pathways are activated response DNA damage, though significance p21–PCNA interaction not completely clear. We analyzed effects expressing miniprotein containing domain upon cell cycle and proliferation various types. compared this with effect mutant form defective PCNA-binding, but retains secondary cyclin–CDK-inhibitory site. No PCNA-dependent were seen short term distribution. However, clonogenic assays show that GFP-peptide can significantly suppress manner. In some types, however, suppression was PCNA-dependent, suggesting environment contributory factor miniprotein. The capacity peptide sequence vivo interest as basis for design potential antiproliferative therapeutic agents.