Retargeting a Dual-Acting sRNA for Multiple mRNA Transcript Regulation

摘要: Multitargeting small regulatory RNAs (sRNAs) represent a potentially useful tool for metabolic engineering applications. Natural multitargeting sRNAs govern bacterial gene expression by binding to the translation initiation regions of protein-coding mRNAs through base pairing. We designed an Escherichia coli based genetic system create and assay dual-acting retargeted-sRNA variants. The variants can be assayed coordinate translational regulation two alternate mRNA leaders fused independent reporter genes. Accordingly, we began with well-characterized E. native DsrA sRNA. merits using include its separation function into independently folded stem-loop domains, wherein alterations at one stem do not necessarily abolish activity other stem. Expression sRNA each was controlled inducer molecules, allowing precise quantification effects sRNA:mRNA interaction in vivo a...