Molecular Cloning of cDNA for a Novel Pea Dof Protein, PsDof1, and Its DNA-Binding Activity to the Promoter of PsDof1 Gene
作者： Hikaru SEKI , Naoki NAKAMURA , Mizuri MARUTANI , Takeharu OKABE , Shiroh SANEMATSU
DOI: 10.5511/PLANTBIOTECHNOLOGY.19.251
关键词: Recombinant DNA 、 Protein family 、 Transcription factor 、 DNA 、 Sp1 transcription factor 、 Complementary DNA 、 Molecular cloning 、 Biology 、 Gene 、 Molecular biology
摘要: A cDNA clone, designated PsDof1, encoding a novel member of the Dof DNA-binding protein family was isolated from pea. Random-binding-site selection and gel retardation experiments showed that GST-PsDof1 recombinant bound to specific DNA sequences with an AAAG core. metal-chelating agent, 1, 10-phenanthroline, abolished binding DNA, but addition Zn2+ restored it, indicating zinc ions are required for its activity. Interestingly, there possible sites PsDof1 in promoter sequence. Indeed, binds AAAG-containing sequence gene higher affinity. These results suggest is transcription factor involved own transcriptional regulation.
elsevier.com
sci-hub.st 参考文章(23)
S. Yanagisawa, K. Izui, Molecular cloning of two DNA-binding proteins of maize that are structurally different but interact with the same sequence motif. Journal of Biological Chemistry. ,vol. 268, pp. 16028- 16036 ,(1993) , 10.1016/S0021-9258(18)82353-5
B Zhang, W Chen, R C Foley, M Büttner, K B Singh, Interactions between distinct types of DNA binding proteins enhance binding to ocs element promoter sequences. The Plant Cell. ,vol. 7, pp. 2241- 2252 ,(1995) , 10.1105/TPC.7.12.2241
Hikaru Seki, Yuki Ichinose, Hisaharu Kato, Tomonori Shiraishi, Tetsuji Yamada, Analysis of cis-regulatory elements involved in the activation of a member of chalcone synthase gene family (PsChs1) in pea Plant Molecular Biology. ,vol. 31, pp. 479- 491 ,(1996) , 10.1007/BF00042222
Robin P. Hyde-DeRuyscher, Ezra Jennings, Thomas Shenk, DNA binding sites for the transcriptional activator/repressor YY1 Nucleic Acids Research. ,vol. 23, pp. 4457- 4465 ,(1995) , 10.1093/NAR/23.21.4457
Yuki Ichinose, Yumiko Hisayasu, Shiroh Sanematsu, Yasuhiro Ishiga, Hikaru Seki, Kazuhiro Toyoda, Tomonori Shiraishi, Tetsuji Yamada, Molecular cloning and functional analysis of pea cDNA E86 encoding homologous protein to hypersensitivity-related hsr203J. Plant Science. ,vol. 160, pp. 997- 1006 ,(2001) , 10.1016/S0168-9452(01)00343-0
B. Zhang, K. B. Singh, ocs element promoter sequences are activated by auxin and salicylic acid in Arabidopsis Proceedings of the National Academy of Sciences of the United States of America. ,vol. 91, pp. 2507- 2511 ,(1994) , 10.1073/PNAS.91.7.2507
Wenqiong Chen, Karam B. Singh, The auxin, hydrogen peroxide and salicylic acid induced expression of the Arabidopsis GST6 promoter is mediated in part by an ocs element Plant Journal. ,vol. 19, pp. 667- 677 ,(1999) , 10.1046/J.1365-313X.1999.00560.X
Shuichi Yanagisawa, Dof DNA-binding proteins contain a novel zinc finger motif Trends in Plant Science. ,vol. 1, pp. 213- 214 ,(1996) , 10.1016/1360-1385(96)86897-X
Montana Mena, Jesus Vicente-Carbajosa, Robert J. Schmidt, Pilar Carbonero, An endosperm-specific DOF protein from barley, highly conserved in wheat, binds to and activates transcription from the prolamin box of a native B-hordein promoter in barley endosperm Plant Journal. ,vol. 16, pp. 53- 62 ,(1998) , 10.1046/J.1365-313X.1998.00275.X
J. L. Riechmann, J. Heard, G. Martin, L. Reuber, C. -Z., Jiang, J. Keddie, L. Adam, O. Pineda, O. J. Ratcliffe, R. R. Samaha, R. Creelman, M. Pilgrim, P. Broun, J. Z. Zhang, D. Ghandehari, B. K. Sherman, G. -L. Yu, Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science. ,vol. 290, pp. 2105- 2110 ,(2000) , 10.1126/SCIENCE.290.5499.2105