Analysis of cis-regulatory elements involved in the activation of a member of chalcone synthase gene family (PsChs1) in pea
作者： Hikaru Seki , Yuki Ichinose , Hisaharu Kato , Tomonori Shiraishi , Tetsuji Yamada
DOI: 10.1007/BF00042222
关键词:
摘要: Cis-regulatory elements involved in the activation of plant defense-related gene encoding chalcone synthase 1 (PsChs1) pea (Pisum sativum L.) were examined by transient transfection, gel mobility shift assay and vitro DNase I-footprinting analysis. Transient transfection revealed that a 61 bp DNA fragment spanning from -242 to -182 PsChs1 was required for maximal promoter activity possibly enhancement elicitor-mediated activation. Nuclear isolate elicitor-treated epicotyl tissues contained some factor(s) specifically bound this form complex with low (LMC, complex) assay. analysis LMC among three protected regions detected fragment, two identical AT-rich sequence, TAAAATACT. Site directed mutation either or both sequences, TAAAATACT TGGAATACT, resulted reduction loss ability LMC. Detailed demonstrated region -226 containing promoter-distal motif imperative PsChs1.
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R. Lois, A. Dietrich, K. Hahlbrock, W. Schulz, A phenylalanine ammonia-lyase gene from parsley: structure, regulation and identification of elicitor and light responsive cis-acting elements. The EMBO Journal. ,vol. 8, pp. 1641- 1648 ,(1989) , 10.1002/J.1460-2075.1989.TB03554.X
M A Lawton, C J Lamb, Transcriptional activation of plant defense genes by fungal elicitor, wounding, and infection. Molecular and Cellular Biology. ,vol. 7, pp. 335- 341 ,(1987) , 10.1128/MCB.7.1.335
Kyoji Yamada, Manabu Matsuda, Yuichi Fujita, Hiroshi Matsubara, Michizo Sugai, A frxC Homolog Exists in the Chloroplast DNAs from Various Pteridophytes and in Gymnosperms Plant and Cell Physiology. ,vol. 33, pp. 325- 327 ,(1992) , 10.1093/OXFORDJOURNALS.PCP.A078258
Testsuji Yamada, Suppression of pisatin, phenylalanine ammonia-lyase mRNA, and chalcone synthase mRNA accumulation by a putative pathogenicity factor from the fungus Mycosphaerella pinodes Molecular Plant-microbe Interactions. ,vol. 2, pp. 256- 261 ,(1989) , 10.1094/MPMI-2-256
Manabu Wada, Hisaharu Kato, Kamal Malik, Permpong Sriprasertsak, Yuki Ichinose, Tomonori Shiraishi, Tetsufi Yamada, A supprescin from a phytopathogenic fungus deactivates transcription of a plant defense gene encoding phenylalanine ammonia-lyase. Journal of Molecular Biology. ,vol. 249, pp. 513- 519 ,(1995) , 10.1006/JMBI.1995.0313
John C. Cushman, Hans J. Bohnert, Salt stress alters A/T-rich DNA-binding factor interactions within the phosphoenolpyruvate carboxylase promoter from Mesembryanthemum crystallinum Plant Molecular Biology. ,vol. 20, pp. 411- 424 ,(1992) , 10.1007/BF00040601
H. Kato, M. Wada, K. Muraya, K. Malik, T. Shiraishi, Y. Ichinose, T. Yamada, Characterization of Nuclear Factors for Elicitor-Mediated Activation of the Promoter of the Pea Phenylalanine Ammonia-Lyase Gene 1 Plant Physiology. ,vol. 108, pp. 129- 139 ,(1995) , 10.1104/PP.108.1.129
Markus Thali, Michael M. Müller, Mauro DeLorenzi, Patrick Matthias, Mariann Bienz, Drosophila homoeotic genes encode transcriptional activators similar to mammalian OTF-2. Nature. ,vol. 336, pp. 598- 601 ,(1988) , 10.1038/336598A0
Yuki Ichinose, Shinji Kawamata, Tetsuji Yamada, ChenCai An, Takeshi Kajiwara, Tomonori Shiraishi, Hachiro Oku, Molecular cloning of chalcone synthase cDNAs from Pisum sativum Plant Molecular Biology. ,vol. 18, pp. 1009- 1012 ,(1992) , 10.1007/BF00019221
Lloyd M. Yu, Christopher J. Lamb, Richard A. Dixon, Purification and biochemical characterization of proteins which bind to the H-box cis-element implicated in transcriptional activation of plant defense genes. Plant Journal. ,vol. 3, pp. 805- 816 ,(1993) , 10.1111/J.1365-313X.1993.00805.X