Heterosandwich immunoswab assay for dengue virus Ns1 antigen detection.

摘要: Dengue and the more severe dengue hemorrhagic fever have been a very critical public health problem globally. Millions of people especially in tropical areas get infected with dengue. An efficient diagnostic is important for early screening infection. In dengue-infected patients, nonstructural protein NS1 present on surface cells secreted plasma. The antigen an target developing quick largely due to its long presence blood. We developed simple-to-use immunoswab-based procedure employing monoclonal antibodies second-generation quadromas. detection limit has established be subnanogram range. assay sensitive, visual end point, also being extremely inexpensive. With this assay, time person would rapid.