Use of the hormone-biosynthesis inhibitors fluridone and paclobutrazol to determine the effects of altered abscisic acid and gibberellin levels on pre-maturity α-amylase formation in wheat grains

摘要: During germination of cereal grain, α-amylase formation is known to be inhibited by abscisic acid (ABA) and stimulated gibberellins (GA). The role these hormones in pre-maturity (PMA) wheat grains less well understood. Our previous work with ABA GA exogenously applied demonstrated a clear stimulatory effect GA, little ABA. Here, glasshouse experiments, fluridone (ABA biosynthesis inhibitor; FD [20 μM]) or paclobutrazol (GA PB were intact, developing the PMA-susceptible variety Rialto at 480 days after anthesis (DAA) assess if reduction endogenous and/or alters PMA formation. experiments conducted under non-PMA-inducing (ambient) PMA-inducing (cold-shock) conditions. In solvent-only treated grains, cold-shock significantly reduced content but increased activity. levels activity ambient conditions, decreased no on either condition. had These results indicate an association between mid-grain development wheat.