Chapter X Isolation, Cultivation and Maintenance of Mycoplasmas

摘要: Publisher Summary This chapter discusses the isolation, cultivation, and maintenance of mycoplasmas that occur in nature as normal flora or pathogens humans, animals, birds. The first isolated mycoplasma, Mycoplasma mycoides , was cultured from cattle suffering contagious bovine pleuropneumonia. binominal name given to organism pleuropneumonia Asterococcus . is derived “fungus form” describes pleomorphic forms seen on microscopy organism. Mycoplasmas are smallest known free-living life. They multiply cell-free culture media, although most species require complex substances, such protein sterols support growth. differ viruses rickettsiae they do not living cells for growth bacteria have no rigid cell wall nor cell-wall mucopeptide its precursors. lack a explains observed when these organisms examined by microscopy.