Growth and division of Escherichia coli under microgravity conditions

摘要: Abstract The growth rate in glucose minimal medium and time of entry into the stationary phase pepton cultures were determined during STS 42 mission space shuttle Discovery. Cells cultured plastic bags was stopped at six different points by lowering temperature to 5°C, a single point, formaldehyde fixation. Based on cell number determination, doubling calculated for flight samples cells shorter (46 min) than ground (59 min). However, larger size expected more rapidly growing not observed volume measurements with electronic particle counter, nor electron microscopic measurement dimensions. Only fixed length percentage constricted found. An optical density increase peptone showed an earlier samples, but this could be confirmed viability counts. sample properties indicative stimulation. taking all observations together, we conclude that microgravity has no effect exponentially Escherichia coli cells.