Nucleotide sequence heterogeneity of an RNA phage population
作者： Esteban Domingo , Donna Sabo , Tadatsugu Taniguchi , Charles Weissmann
DOI: 10.1016/0092-8674(78)90223-4
关键词: Nucleic acid sequence 、 Molecular biology 、 Biology 、 Oligonucleotide 、 RNA 、 Genetics 、 Population 、 Sequence (medicine) 、 Gene 、 Genome 、 Wild type 、 General Biochemistry, Genetics and Molecular Biology
摘要: The nucleotide sequence of 32P-RNA from Q beta phage clones was sampled by two-dimensional polyacrylamide gel electrophoresis the RNAase T1-resistant oligonucleotides (T1 fingerprinting). About 15% derived a multiply passaged population showed fingerprint patterns which deviated that RNA total population. All deviations examined could be attributed to one and, less frequently, two or more transitions. Since fingerprinting technique allows analysis only about 10% sequence, we estimate each viable genome in differs positions "average" parental Several deviant were tested growth competition against "wildtype" population, after 10-20 generations, resulting "wild-type" T1 pattern. We propose is dynamic equilibrium, with mutants arising at high rate (Batschelet, Domingo and Weissmann, 1976; Domingo, Flavell 1976) on hand, being strongly selected other. cannot described as defined unique structure, but rather weighted average large number different individual sequences.
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