Assessment of bacterial superficial contamination in classical or ritually slaughtered cattle using metagenetics and microbiological analysis.
作者： N. Korsak , B. Taminiau , C. Hupperts , L. Delhalle , C. Nezer
DOI: 10.1016/J.IJFOODMICRO.2016.10.013
关键词: Actinobacteria phylum 、 Biology 、 Rump 、 Microbiology 、 Veterinary medicine 、 Bacterial population 、 Contamination 、 Firmicutes 、 Pyrosequencing 、 Operational taxonomic unit 、 Hygiene
摘要: The aim of this study was to investigate the influence slaughter technique (Halal vs Classical slaughter) on superficial contamination cattle carcasses, by using traditional microbiological procedures and 16S rDNA metagenetics. purpose also neck area identify bacteria originating from digestive or respiratory tract. Twenty bovine carcasses (10 each group) were swabbed at slaughterhouse, where both slaughtering methods are practiced. Two swabbing areas chosen: one "legal" zone 1600cm2 (composed zones rump, flank, brisket forelimb) locally (200cm2). Samples submitted classical microbiology for aerobic Total Viable Counts (TVC) 30°C Enterobacteriaceae counts, while metagenetic analysis performed same samples. results revealed no significant differences between practices; with values 3.95 4.87log CFU/100cm2 0.49 1.94log CFU/100cm2, TVC respectively. Analysis pyrosequencing data showed that in bacterial population abundance mainly observed compared area. Bacterial genera belonging Actinobacteria phylum more abundant "Halal" samples, Brevibacterium Corynebacterium encountered all areas. This case Firmicutes populations (families Aerococcaceae, Planococcaceae). Except Planococcoceae, Operational Taxonomic Unit (OTU) abundances tract groups. In conclusion, method does not pattern terms specific markers However, precise taxonomy genus level highlights Although clearly proven study, hygiene practices used during protocols could explain
core.ac.uk
sci-hub.se 参考文章(39)
Y. GHAFIR, B. CHINA, K. DIERICK, L. DE ZUTTER, G. DAUBE, Hygiene indicator microorganisms for selected pathogens on beef, pork, and poultry meats in Belgium. Journal of Food Protection. ,vol. 71, pp. 35- 45 ,(2008) , 10.4315/0362-028X-71.1.35
Changyong Lyu, Chaoyin Chen, Feng Ge, Diqiu Liu, Shenglan Zhao, Dan Chen, A preliminary metagenomic study of puer tea during pile fermentation Journal of the Science of Food and Agriculture. ,vol. 93, pp. 3165- 3174 ,(2013) , 10.1002/JSFA.6149
T Yesildere, H Aksu, O Erdogan, C Mason, SI Hughes, JL McKinstry, MH Anil, E Matur, Comparison of religious slaughter of sheep with methods that include pre-slaughter stunning, and the lack of differences in exsanguination, packed cell volume and meat quality parameters Animal Welfare. pp. 387- 392 ,(2004)
E Matur, T Yesildere, H Aksu, O Erdogan, C Mason, SI Hughes, MH Anil, JL McKinstry, HR Weaver, Comparison of Halal slaughter with captive bolt stunning and neck cutting in cattle: exsanguination and quality parameters Animal Welfare. ,vol. 15, pp. 325- 330 ,(2006)
SHERYL M. AVERY, CAROL-ANN REID, DOUGLAS WILSON, MARY HOWELL, ALEXANDER M. JOHNSTON, SAVA BUNCIC, MICHAEL L. HUTCHISON, LISA D. WALTERS, A comparison of wet-dry swabbing and excision sampling methods for microbiological testing of bovine, porcine, and ovine carcasses at red meat slaughterhouses. Journal of Food Protection. ,vol. 68, pp. 2155- 2162 ,(2005) , 10.4315/0362-028X-68.10.2155
Cristina Rodriguez, Bernard Taminiau, Bastien Brévers, Véronique Avesani, Johan Van Broeck, Aurélia Leroux, Marjorie Gallot, Antoine Bruwier, Hélene Amory, Michel Delmée, Georges Daube, Faecal microbiota characterisation of horses using 16 rdna barcoded pyrosequencing, and carriage rate of clostridium difficile at hospital admission BMC Microbiology. ,vol. 15, pp. 181- 181 ,(2015) , 10.1186/S12866-015-0514-5
Ji Young Jung, Se Hee Lee, Jeong Myeong Kim, Moon Su Park, Jin-Woo Bae, Yoonsoo Hahn, Eugene L. Madsen, Che Ok Jeon, Metagenomic Analysis of Kimchi, a Traditional Korean Fermented Food† Applied and Environmental Microbiology. ,vol. 77, pp. 2264- 2274 ,(2011) , 10.1128/AEM.02157-10
Trine Hansen, Beate Skånseng, Jeffrey Hoorfar, Charlotta Löfström, Evaluation of Direct 16S rDNA Sequencing as a Metagenomics-based Approach to Screening Bacteria in Bottled Water Biosecurity and Bioterrorism-biodefense Strategy Practice and Science. ,vol. 11, ,(2013) , 10.1089/BSP.2012.0073
J.M. McEvoy, J.J. Sheridan, I.S. Blair, D.A. McDowell, Microbial contamination on beef in relation to hygiene assessment based on criteria used in EU Decision 2001/471/EC International Journal of Food Microbiology. ,vol. 92, pp. 217- 225 ,(2004) , 10.1016/J.IJFOODMICRO.2003.09.010
Ludmila Chistoserdova, Recent progress and new challenges in metagenomics for biotechnology Biotechnology Letters. ,vol. 32, pp. 1351- 1359 ,(2010) , 10.1007/S10529-010-0306-9