Preparation of Nano‐ and Microcapsules by Electrophoretic Polymer Assembly

摘要: Nanoand microcapsules are of significant interest for application in biomedicine, especially diagnostics and therapeutic delivery. The fabrication polymer capsules is often accomplished by the particle-mediated assembly films, whereby films formed on particles through polymerization or depositing multiple layers layer-by-layer (LbL) assembly. In particular, LbL enables use various types, shapes, sizes as templates a suite polymers with different properties (functionality, responsiveness, degradability) to be used engineer physical, chemical, biological capsules. versatility particle has led development range coated diverse applications, including drug vaccine 4a–f] Despite progress field LbLengineered particles, film-deposition process typically requires numerous centrifugation rinse steps separate coating material (e.g., polymer) particles. Furthermore, it generally limited either dense and/or large enough centrifugal sedimentation. Other methods generate have also been reported: example, atomization techniques, filter membranes, microfluidic systems used; however, each these approaches reduces diversity types that can employed. These limitations highlight necessity alternative rapid robust formation LbLassembled coatings engineered core– shell Herein, we report an electrophoretic (EPA) technique sizes. this approach, electrophoresis and, following core removal, multilayer An inherent requirement EPA immobilization porous hydrogel; study, namely, biologically derived polysaccharide agarose. Agarose historically biopolymers proteins nucleic acids) because gelates under ambient conditions, shows low reactivity, easy prepare, various, tunable pore present system, agarose acts “natural immobilizing system”. However, did not simply free from deposited polymer, but deposit immobilized (Figure 1). This handling times, minimizes