Approaches to quantifying and visualizing polyelectrolyte multilayer film formation on particles.

摘要: Colloidal particles prepared by using the layer-by-layer technique are increasingly finding application in diagnostics, drug delivery, and sensing. Herein, we outline methods for applying three established techniques, confocal laser scanning microscopy (CLSM), flow cytometry, differential interference contrast (DIC) microscopy, to characterize ultrathin films of poly(styrenesulfonate) (PSS) poly(allylamine hydrochloride) (PAH) assembled on silica particles. Both CLSM cytometry require use fluorescently labeled polyelectrolytes (PEs). The film homogeneity can be assessed CLSM, while allows analysis at unparalleled speed (thousands per second) with unprecedented sensitivity (<0.5 fg adsorbed polymer) polydispersed different size (∼300 nm tens micrometers). Using measurements, conjunction quartz crystal microgravimetry measurements planar supports, quantification PSS/PAH layer build...