miR-29b enhances prostate cancer cell invasion independently of MMP-2 expression.

摘要: The ability to metastasize is one of the most important characteristics neoplastic cells. An imbalance between action some matrix metalloproteinases (MMPs) and tissue inhibitors MMPs drives invasion process. Some studies have suggested that MMP-2 involved in metastasis, while other reported collagen production by cancer cells might also contribute motility. However, decreased expression microRNA-29b (miR-29b), which may control gene expression, has been shown prostate (PCa). objectives present study were clarify whether as well collagens I III (encoded COL1A1 COL3A1, respectively) are controlled miR-29b determine metastasis altered this relationship. PCa DU145 PC-3 transfected with 100 μL OPTI-MEM containing 100 nmol (or its inhibitor) along 1.5 μL lipofectamine. Positive negative controls prepared using same protocol. MMP-2, COL3A1 messenger RNA (mRNA) levels evaluated via real-time polymerase chain reaction (qRT-PCR). For qRT-PCR, 6 × 104 cells used. Invasion conducted Matrigel assays, simulate extracellular After transfection 3 × 104 cells, was allowed proceed for 48 h. Invasive counted under an optical microscope. Each experiment performed triplicate. mRNA not expressed after miR-29b. inhibitor, (p = 0.02) (p = 0.06) increased a large number PC3 invaded membrane. In vitro showed reducing amount lead higher cell process independent MMP-2. Collagen miR-29b, facilitate motility Thus, suggests plays active role restoration decrease metastasis. Altogether, these findings support further exploration drug therapy targeting aspect circuit.