Binding of stallion spermatozoa to the equine zona pellucida after coculture with oviductal epithelial cells.

摘要: The objective of this study was to determine whether coculture stallion spermatozoa and mare oviductal (uterine tubal) epithelial cells induced sperm cell capacitation in vitro. Capacitation as determined by zona binding chlortetracycline staining the compared for spermatozoa: (1) incubated with medium alone (negative control), (2) treated calcium ionophore A23187 (positive control) or (3) cultured (OEC) 4 h. Chlortetracycline patterns bound zonae were used group uncapacitated, capacitated acrosome reacted. attached stained evaluation after initial (pulse) 1 h co-incubation (chase). More OEC treatments at both pulse chase than control (P < 0.001). pulse, reacted chase, co-culture groups controls Staining not pellucida each differed 0.05) from population that pellucida. There a higher percentage lower acrosome-reacted represented treatment suspensions cells.(ABSTRACT TRUNCATED AT 250 WORDS)