Vibrational spectroscopy in sensing radiobiological effects: analyses of targeted and non-targeted effects in human keratinocytes

摘要: Modern models of radiobiological effects include mechanisms damage initiation, sensing and repair, for those cells that directly absorb ionizing radiation as well experience molecular signals from irradiated cells. In the former case, are termed targeted while, in latter, non-targeted effects. It has emerged phenomena occur at low doses below 1 Gy associated with cell-cycle-dependent DNA repair. Likewise bystander-irradiated effect saturates 0.5 Gy. Both these challenge limits detection vibrational spectroscopy. this paper, a study both HaCaT human keratinocytes gamma ray photons is conducted case cells, it shown cell line does exhibit hyperradiosensitivity increased radioresistance doses, transition between two occurring dose 200 mGy, survival other physiological function follow induced repair model. Raman FTIR signatures to similar model, suggesting spectra pro- anti-apoptotic signalling ROS were inhibited mitogen-activated protein kinase (MAPK) transduction pathway. spectral profiles correlated markers bystander transduction. This work demonstrates first time detected by spectroscopy vitro.