cAMP-dependent kinases in the algal flagellate Euglena gracilis.
作者： Isabelle A Carre , LN Edmunds
DOI: 10.1016/S0021-9258(18)45853-X
关键词: Mutant 、 Euglena gracilis 、 Biochemistry 、 Euglena 、 Kinase 、 Binding protein 、 Biology 、 Protein kinase A 、 Kinase activity 、 Phosphorylation
摘要: Euglena cells grown in diurnal light-dark cycles exhibit circadian variations of their cAMP content, which we believe to be under the control an endogenous timer because they persist constant darkness absence any environmental time cue. We think that these oscillations may play a role regulation some numerous cellular activities are known display rhythmicities this organism. The algal is still controversial, however, since nature "receptor" unknown. show extracts achlorophyllous ZC mutant gracilis contain two cAMP-binding proteins, bind with high affinity (Kd values 10 nM and 30 nM) can separated by DEAE-cellulose chromatography. Protein kinase activity was assayed using Kemptide as substrate. Stimulation observed after partial purification Two peaks were resolved, corresponding distinct enzymes different cAMP-analog specificities. Thus, signaling plant proceed phosphorylation target proteins cAMP-dependent kinases, manner similar animal cells.
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