Seasonal Assessment of Opportunistic Premise Plumbing Pathogens in Roof-Harvested Rainwater Tanks

作者: Kerry A. Hamilton , Warish Ahmed , Andrew Palmer , Kylie Smith , Simon Toze

DOI: 10.1021/ACS.EST.6B04814

关键词: AcanthamoebaDry seasonLegionella pneumophilaBiologyWet seasonIndicator bacteriaMicrobiologyRainwater harvestingLegionellaFeces

摘要: A seasonal study on the occurrence of six opportunistic premise plumbing pathogens (OPPPs) in 24 roof-harvested rainwater (RHRW) tanks repeatedly sampled over monthly sampling events (n = 144) from August 2015 to March 2016 was conducted using quantitative qPCR. Fecal indicator bacteria (FIB) Escherichia coli (E. coli) and Enterococcus spp. were enumerated culture-based methods. All tank water samples positive for at least one OPPP (Legionella spp., Legionella pneumophila, Mycobacterium avium, intracellulare, Pseudmonas aeruginosa, or Acanthamoeba spp.) during entire course study. FIB positively but weakly correlated with P. aeruginosa vs τ 0.090, p 0.027; 0.126, 0.002), not other OPPPs. FIBs more prevalent wet season than dry season, L. pneumophila only observed season. However, concentrations M. intra...

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