The IS10 transposase mRNA is destabilized during antisense RNA control.
作者： C.C. Case , E.L. Simons , R.W. Simons
DOI: 10.1002/J.1460-2075.1990.TB08234.X
关键词: Messenger RNA 、 Gene expression 、 Cell biology 、 RNA Stability 、 Ribosome 、 Transcription (biology) 、 RNA 、 Molecular biology 、 Antisense RNA 、 Ribonuclease III 、 Biology
摘要: RNA stability is an important component of gene expression, and antisense RNAs have been proposed to alter target stability. We show here that the IS10 transposase mRNA, RNA-IN, rendered unstable during control by RNA, RNA-OUT. Destabilization requires RNA-OUT/RNA-IN pairing ribonuclease III cleavage. Independent such cleavage, RNA-OUT through disruption its secondary structure. Pairing has no other obvious effects on RNA-IN transcription or Nevertheless, destabilization not required for in vivo. In accompanying paper [Ma,C. Simons, R.W. (1990) EMBO J., 9, 1267-1274 we blocks ribosome binding RNA-IN. Were it at this level, would play a more prominent role.
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