Novel insights into the regulation of skeletal muscle protein synthesis as revealed by a new nonradioactive in vivo technique

摘要: In this study, the principles of surface sensing translation (SUnSET) were used to develop a nonradioactive method for ex vivo and in measurements protein synthesis (PS). Compared with controls, we first demonstrate excellent agreement between SUnSET [(3)H]phenylalanine when detecting synergist ablation-induced increases skeletal muscle PS vivo. We then show that can detect same increase (IV-SUnSET). addition, IV-SUnSET detected food deprivation-induced decreases heart, kidney, muscles, similar changes being visualized an immunohistochemical version (IV-IHC-SUnSET). By combining IV-IHC-SUnSET transfection, constitutively active PKB induces robust PS. Furthermore, transfection Ras homolog enriched brain (Rheb) revealed PKB-independent activation mammalian target rapamycin is also sufficient induce Finally, exposed existence fiber type-dependent differences PS, type 2B 2X fibers significantly lower than 2A within muscle. Thus, our allowed us accurately visualize quantify under various conditions novel insights into regulation