Measurement of the protein-synthetic activity in vivo of various tissues in rats by using [3H]Puromycin.

摘要: The validity of a new technique was examined for estimating the protein-synthetic activity various tissues in vivo. basic assumption underlying method is that number peptide chains growing on each active ribosome would increase as tissue increases. principle procedure, which devised originally by Wool & Kurihara [(1967) Proc. Natl. Acad. Sci. U.S.A. 58, 2401-2407] to determine vitro functional ribosomes skeletal muscle, follows. Puromycin known bind easily C-terminal end and thus stop further chain elongation. Hence, if puromycin molecules attached nascent determined using radioactive tracer, one can estimate peptides, i.e. protein synthesis. By this technique, it shown both starvation feeding protein-free diet caused marked decreases relative rate formation peptidyl-puromycin, synthesis liver, heart, spleen, testis, lung, kidney intestine.