Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites.

摘要: Head and neck cancer (HNC) is a heterogeneous malignant disease with distinct global distribution. Metabolic adaptations of HNC are significantly gaining clinical interests nowadays. Here, we investigated effects on differential expression volatile metabolites in human saliva. We applied headspace solid phase microextraction coupled gas chromatography-mass spectrometry analysis saliva samples collected from 59 subjects (HNC − 32, Control 27). identified quantified 48 organic (VOMs) observed profound these metabolites. These were VOM specific differed the biologically comparable healthy controls. induced changes salivary composition well attributed to vivo metabolic effects. A panel 15 VOMs variable importance projection (VIP) score >1, false discovery rate (FDR) corrected p-value < 0.05 log2 fold change (log2 FC) value ≥0.58/≤−0.58 regarded as discriminatory pathophysiological importance. Afterwards, receiver operator characteristic curve (ROC) projected certain viz., 1,4-dichlorobenzene, 1,2-decanediol, 2,5-bis1,1-dimethylethylphenol E-3-decen-2-ol highest class segregation potential. Moreover, pathways portrayed several dysregulated HNC, which enhanced our basic understanding changes. Our observations could redefine known/already systemic phenomenons (e.g. biochemical pathways). findings will inspire further research this direction may open unconventional avenues for non-invasive monitoring its therapy future.