Stoichiometry of estramustine phosphate binding to MAP2 measured by the disassembly of chick brain MAP2: Tubulin microtubules
作者： Roy G. Burns
DOI: 10.1002/CM.970170304
关键词: Biochemistry 、 Microtubule 、 Tubulin 、 Binding site 、 Biology 、 Mechanism of action 、 Protein concentration 、 Estramustine phosphate 、 Stoichiometry 、 Microtubule-associated protein 、 Cell biology 、 Structural biology
摘要: The concentration of estramustine phosphate required to inhibit the assembly or induce disassembly chick brain MAP2:tubulin microtubules is markedly dependent upon microtubule protein concentration. Analysis this relationship shows that and tubulin compete for common MAP2 sites, can bind 5-6 moles.mole-1 phosphate, Kd these sites congruent 20 microM phosphate. It proposed two molecules interact with each three tubulin-binding interaction by neutralising highly conserved basic residues.
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