Optimization of environmental factors for improved production of rhamnolipid biosurfactant by Pseudomonas aeruginosa RS29 on glycerol
作者： Rashmi Rekha Saikia , Suresh Deka , Manab Deka , Hemen Sarma
关键词: Ammonium nitrate 、 Microbiology 、 Fatty acid 、 Biomass 、 Potassium nitrate 、 Chemistry 、 Salinity 、 Sodium 、 Rhamnolipid 、 Incubation 、 Food science
摘要: A biosurfactant producing Pseudomonas aeruginosa RS29 (identified on the basis of 16S rDNA analysis) with good foaming and emulsification properties has been isolated from crude oil contaminated sites. Optimization different environmental factors was carried out an objective to achieve maximum production biosurfactant. Production estimated in terms surface tension reduction (E24) index. It recorded that strain produced highest after 48 h incubation at 37.5 °C, a pH range 7–8 salinity <0.8% (w/v). Ammonium nitrate used experiment best nitrogen source for growth biomass P. RS29. On other hand sodium potassium enhanced (Surface tension, 26.3 26.4 mN/m E24 index, 80 79% respectively). The CMC 90 mg/l. Maximum (6.30 g/l) (0.80 were optimal C/N ratio 12.5. Biochemical analysis FTIR spectra confirmed rhamnolipid nature. GC-MS revealed presence C8 C10 fatty acid components purified (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
sci-hub.se 参考文章(52)
L. M. Santa Anna, G. V. Sebastian, N. Pereira, T. L. M. Alves, E. P. Menezes, D. M. G. Freire, Production of biosurfactant from a new and promising strain of Pseudomonas aeruginosa PA1. Applied Biochemistry and Biotechnology. ,vol. 91, pp. 459- 467 ,(2001) , 10.1007/978-1-4612-0217-2_39
Bachar H. Hassan, John E. Cronan, Protein-Protein Interactions in Assembly of Lipoic Acid on the 2-Oxoacid Dehydrogenases of Aerobic Metabolism Journal of Biological Chemistry. ,vol. 286, pp. 8263- 8276 ,(2011) , 10.1074/JBC.M110.194191
Mahmoud Abouseoud, Aziza Yataghene, Abdeltif Amrane, Rachida Maachi, Biosurfactant production by free and alginate entrapped cells of Pseudomonas fluorescens Journal of Industrial Microbiology & Biotechnology. ,vol. 35, pp. 1303- 1308 ,(2008) , 10.1007/S10295-008-0411-0
S. Harvey, I. Elashvili, J. J. Valdes, D. Kamely, A. M. Chakrabarty, Enhanced Removal of Exxon Valdez Spilled Oil from Alaskan Gravel by a Microbial Surfactant Nature Biotechnology. ,vol. 8, pp. 228- 230 ,(1990) , 10.1038/NBT0390-228
M. Benincasa, A. Abalos, I. Oliveira, A. Manresa, Chemical structure, surface properties and biological activities of the biosurfactant produced by Pseudomonas aeruginosa LBI from soapstock Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. ,vol. 85, pp. 1- 8 ,(2004) , 10.1023/B:ANTO.0000020148.45523.41
T. C. Jenkins, E. J. Thies, E. E. Mosley, Direct methylation procedure for converting fatty amides to fatty acid methyl esters in feed and digesta samples. Journal of Agricultural and Food Chemistry. ,vol. 49, pp. 2142- 2145 ,(2001) , 10.1021/JF001356X
N.K. Bordoloi, B.K. Konwar, Bacterial biosurfactant in enhancing solubility and metabolism of petroleum hydrocarbons. Journal of Hazardous Materials. ,vol. 170, pp. 495- 505 ,(2009) , 10.1016/J.JHAZMAT.2009.04.136
Zulfiqar Ali Raza, Muhammad Saleem Khan, Zafar M Khalid, None, Evaluation of distant carbon sources in biosurfactant production by a gamma ray-induced Pseudomonas putida mutant Process Biochemistry. ,vol. 42, pp. 686- 692 ,(2007) , 10.1016/J.PROCBIO.2006.10.001
K. Venkata Ramana, N. G. Karanth, Factors affecting biosurfactant production using Pseudomonas aeruginosa CFTR‐6 under submerged conditions Journal of Chemical Technology & Biotechnology. ,vol. 45, pp. 249- 257 ,(2007) , 10.1002/JCTB.280450402
Michele I. Van Dyke, Hung Lee, Jack T. Trevors, Applications of microbial surfactants Biotechnology Advances. ,vol. 9, pp. 241- 252 ,(1991) , 10.1016/0734-9750(91)90006-H