Development of Infrared Imaging to Measure Thermogenesis in Cell Culture: Thermogenic Effects of Uncoupling Protein-2, Troglitazone, and β-Adrenoceptor Agonists

作者: Mark A. Paulik , Richard G. Buckholz , Mary E. Lancaster , Walter S. Dallas , Emily A. Hull‐Ryde

DOI: 10.1023/A:1011993019385

关键词: Uncoupling proteinEndocrinologyRotenoneLipolysisCell cultureTroglitazoneThermogenesisInternal medicineAdipocyteBiologyCycloheximide

摘要: Purpose. Although the effects of thermogenic agents in cell culture can be measured by direct microcalorimetry, only a few samples can be analyzed over several hours. In this report, …

参考文章(23)
Steven A Kliewer, Mark A Paulik, Kelli D Plunket, Jurgen M Lehmann, James E Weiel, James M Lenhard, Effects of troglitazone and metformin on glucose and lipid metabolism: alterations of two distinct molecular pathways. Biochemical Pharmacology. ,vol. 54, pp. 801- 808 ,(1997) , 10.1016/S0006-2952(97)00229-3
S Rehnmark, M Néchad, D Herron, B Cannon, J Nedergaard, Alpha- and beta-adrenergic induction of the expression of the uncoupling protein thermogenin in brown adipocytes differentiated in culture. Journal of Biological Chemistry. ,vol. 265, pp. 16464- 16471 ,(1990) , 10.1016/S0021-9258(17)46245-4
Wen-Jei Yang, Paul P. T. Yang, Literature survey on biomedical applications of thermography. Bio-medical Materials and Engineering. ,vol. 2, pp. 7- 18 ,(1992) , 10.3233/BME-1992-2102
D. Ricquier, L. Casteilla, F. Bouillaud, Molecular studies of the uncoupling protein. The FASEB Journal. ,vol. 5, pp. 2237- 2242 ,(1991) , 10.1096/FASEBJ.5.9.1860614
M Lafontan, M Berlan, Fat cell adrenergic receptors and the control of white and brown fat cell function Journal of Lipid Research. ,vol. 34, pp. 1057- 1091 ,(1993) , 10.1016/S0022-2275(20)37695-1
J. Nedergaard, B. Cannon, [3H]GDP binding and thermogenin amount in brown adipose tissue mitochondria from cold-exposed rats American Journal of Physiology-cell Physiology. ,vol. 248, ,(1985) , 10.1152/AJPCELL.1985.248.3.C365
Martin Reers, Thomas W. Smith, Lan Bo Chen, J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential. Biochemistry. ,vol. 30, pp. 4480- 4486 ,(1991) , 10.1021/BI00232A015
Hartfried Böttcher, Peter Fürst, A new microcatheter enables microcalorimetric assessment of thermogenesis after stimulation in cell cultures. Journal of Biochemical and Biophysical Methods. ,vol. 32, pp. 191- 194 ,(1996) , 10.1016/0165-022X(96)00010-3
PHILIP J. SCARPACE, Thermoregulation with Age: Role of Beta-Adrenergic Signal Transduction Annals of the New York Academy of Sciences. ,vol. 813, pp. 111- 116 ,(1997) , 10.1111/J.1749-6632.1997.TB51680.X
J. Enrique Silva, Peggy S. Matthews, Full expression of uncoupling protein gene requires the concurrence of norepinephrine and triiodothyronine. Molecular Endocrinology. ,vol. 2, pp. 706- 713 ,(1988) , 10.1210/MEND-2-8-706