Langmuir-Blodgett film based on MEH-PPV for cholesterol biosensor.
作者： Zimple Matharu , Sunil K. Arya , S.P. Singh , Vinay Gupta , B.D. Malhotra
DOI: 10.1016/J.ACA.2008.12.023
关键词: Atomic force microscopy 、 Differential pulse voltammetry 、 Analytical chemistry 、 Chemistry 、 Detection limit 、 Cholesterol oxidase 、 Langmuir–Blodgett film 、 Contact angle 、 Stearic acid 、 Biosensor
摘要: Cholesterol oxidase (ChOx) has been immobilized onto conducting poly[2-methoxy,5-(2′-ethyl-hexyloxy)-1,4-phenylene vinylene] (MEH-PPV)/stearic acid (SA) Langmuir–Blodgett film transferred octadecanethiol (ODT) modified gold plate. The ChOx/MEH-PPV/SA LB bioelectrode exhibits characterized by FT-IR, contact angle, and atomic force microscopy. response of the carried out using differential pulse voltammetry (DPV) studies reveal linearity from 1.29 to 12.91 mM cholesterol concentration time as 30 s. This values correlation coefficient 0.9939, standard deviation 0.0029 μA limit detection 1.66 mM. UV–visible spectrophotometer that 5.2 × 10−3 U ChOx are actively working per cm2 area this is thermally stable upto 55 °C with reusability about 60 times.
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Anjan Kumar Basu, Parimal Chattopadhyay, Utpal Roychoudhuri, Runu Chakraborty, Development of cholesterol biosensor based on immobilized cholesterol esterase and cholesterol oxidase on oxygen electrode for the determination of total cholesterol in food samples Bioelectrochemistry. ,vol. 70, pp. 375- 379 ,(2007) , 10.1016/J.BIOELECHEM.2006.05.006
Rahul Singhal, W. Takashima, K. Kaneto, S.B. Samanta, S. Annapoorni, B.D. Malhotra, Langmuir–Blodgett films of poly(3-dodecyl thiophene) for application to glucose biosensor Sensors and Actuators B-chemical. ,vol. 86, pp. 42- 48 ,(2002) , 10.1016/S0925-4005(02)00145-4
A KUMAR, R PANDEY, B BRANTLEY, Tetraethylorthosilicate film modified with protein to fabricate cholesterol biosensor Talanta. ,vol. 69, pp. 700- 705 ,(2006) , 10.1016/J.TALANTA.2005.11.003
Suman Singh, Pratima R. Solanki, M.K. Pandey, B.D. Malhotra, Cholesterol biosensor based on cholesterol esterase, cholesterol oxidase and peroxidase immobilized onto conducting polyaniline films Sensors and Actuators B: Chemical. ,vol. 115, pp. 534- 541 ,(2006) , 10.1016/J.SNB.2005.10.025
Jie Shen, Chung-Chiun Liu, Development of a screen-printed cholesterol biosensor: Comparing the performance of gold and platinum as the working electrode material and fabrication using a self-assembly approach Sensors and Actuators B-chemical. ,vol. 120, pp. 417- 425 ,(2007) , 10.1016/J.SNB.2006.02.035
Zimple Matharu, G. Sumana, Sunil K. Arya, S. P. Singh, Vinay Gupta, B. D. Malhotra, Polyaniline Langmuir-Blodgett film based cholesterol biosensor. Langmuir. ,vol. 23, pp. 13188- 13192 ,(2007) , 10.1021/LA702123A
Jianping Li, Tuzhi Peng, Yuqiang Peng, A Cholesterol Biosensor Based on Entrapment of Cholesterol Oxidase in a Silicic Sol‐Gel Matrix at a Prussian Blue Modified Electrode Electroanalysis. ,vol. 15, pp. 1031- 1037 ,(2003) , 10.1002/ELAN.200390124
A. Parra, E. Casero, F. Pariente, L. Vázquez, E. Lorenzo, Cholesterol oxidase modified gold electrodes as bioanalytical devices Sensors and Actuators B-chemical. ,vol. 124, pp. 30- 37 ,(2007) , 10.1016/J.SNB.2006.11.051
Yoon-Chae Nah, Seok-Soon Kim, Jeong-Ho Park, Hong-Ju Park, Jang Jo, Dong-Yu Kim, Enhanced electrochromic absorption in Ag nanoparticle embedded conjugated polymer composite films Electrochemistry Communications. ,vol. 9, pp. 1542- 1546 ,(2007) , 10.1016/J.ELECOM.2007.02.009
Manoj Ku Ram, Nabin Sarkar, Paolo Bertoncello, Anjana Sarkar, Riccardo Narizzano, Claudio Nicolini, Fabrication and characterization of poly[(2-methoxy-5-(2′-ethyl-hexyloxy)phenylene vinylene] (MEH-PPV) Langmuir–Schaefer films and their application as photoelectrochemical cells Synthetic Metals. ,vol. 122, pp. 369- 378 ,(2001) , 10.1016/S0379-6779(00)00396-9