Development of a screen-printed cholesterol biosensor: Comparing the performance of gold and platinum as the working electrode material and fabrication using a self-assembly approach
作者： Jie Shen , Chung-Chiun Liu
DOI: 10.1016/J.SNB.2006.02.035
关键词:
摘要: Abstract Gold (Au) and platinum (Pt) were used as the working electrode material to detect cholesterol in solution through enzymatically generated hydrogen peroxide (H2O2). Both gold capable of detecting electrochemical oxidation H2O2, could be material. By comparison, however, Au was preferable over Pt terms higher response current better sensitivity. Therefore, chosen for fabrication a thick-film screen-printed biosensor consisting three electrodes on an alumina substrate (working: Au, reference: Ag/AgCl, counter: Au). The immobilization enzyme oxidase (ChOx, E.C. 1.1.3.6) achieved using self-assembly approach. A thiol, 3-mercaptopropionic acid (MPA), self-assembled onto forming thin organic layer that served anchor immobilization. 1-Ethyl-3(3-dimethylamino propyl)carbodiimide methiodide (EDC) then immobilize ChOx covalently carbodiimide coupling between carboxyl (–COOH) groups MPA amino (–NH2) enzyme. Electrochemical measurements showed this responded well cholesterol, confirming method effective. reproducibility, interference, storage stability studied assessed.
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sci-hub.se 参考文章(31)
Toshio Yao, Kazuyoshi Takashima, Amperometric biosensor with a composite membrane of sol-gel derived enzyme film and electrochemically generated poly(1,2-diaminobenzene) film. Biosensors and Bioelectronics. ,vol. 13, pp. 67- 73 ,(1998) , 10.1016/S0956-5663(97)00076-6
Victoria Shumyantseva, Gilda Deluca, Tatiana Bulko, Sandro Carrara, Claudio Nicolini, Sergei A. Usanov, Alexander Archakov, Cholesterol amperometric biosensor based on cytochrome P450scc. Biosensors and Bioelectronics. ,vol. 19, pp. 971- 976 ,(2004) , 10.1016/J.BIOS.2003.09.001
J. C. Vidal, E. Garcia-Ruiz, J. Espuelas, T. Aramendia, J. R. Castillo, Comparison of biosensors based on entrapment of cholesterol oxidase and cholesterol esterase in electropolymerized films of polypyrrole and diaminonaphthalene derivatives for amperometric determination of cholesterol. Analytical and Bioanalytical Chemistry. ,vol. 377, pp. 273- 280 ,(2003) , 10.1007/S00216-003-2120-X
S.P Martin, D.J Lamb, J.M Lynch, S.M Reddy, Enzyme-based determination of cholesterol using the quartz crystal acoustic wave sensor Analytica Chimica Acta. ,vol. 487, pp. 91- 100 ,(2003) , 10.1016/S0003-2670(03)00504-X
Jianping Li, Tuzhi Peng, Yuqiang Peng, A Cholesterol Biosensor Based on Entrapment of Cholesterol Oxidase in a Silicic Sol‐Gel Matrix at a Prussian Blue Modified Electrode Electroanalysis. ,vol. 15, pp. 1031- 1037 ,(2003) , 10.1002/ELAN.200390124
Shannon Snellings, Jason Fuller, Matthew Pitner, David W Paul, An acoustic wave biosensor for human low-density lipoprotein particles: construction of selective coatings. Biosensors and Bioelectronics. ,vol. 19, pp. 353- 363 ,(2003) , 10.1016/S0956-5663(03)00192-1
Arun Kumar, Rajesh Malhotra, B.D. Malhotra, S.K. Grover, Co-immobilization of cholesterol oxidase and horseradish peroxidase in a sol–gel film Analytica Chimica Acta. ,vol. 414, pp. 43- 50 ,(2000) , 10.1016/S0003-2670(00)00792-3
Manli Guo, Jinhua Chen, Jia Li, Lihua Nie, Shouzhuo Yao, Carbon Nanotubes-Based Amperometric Cholesterol Biosensor Fabricated Through Layer-by-Layer Technique Electroanalysis. ,vol. 16, pp. 1992- 1998 ,(2004) , 10.1002/ELAN.200403053
K Vengatajalabathy Gobi, Fumio Mizutani, None, Layer-by-layer construction of an active multilayer enzyme electrode applicable for direct amperometric determination of cholesterol Sensors and Actuators B-chemical. ,vol. 80, pp. 272- 277 ,(2001) , 10.1016/S0925-4005(01)00911-X
Xiao Jun Wu, Martin M. F. Choi, Hydrogel Network Entrapping Cholesterol Oxidase and Octadecylsilica for Optical Biosensing in Hydrophobic Organic or Aqueous Micelle Solvents Analytical Chemistry. ,vol. 75, pp. 4019- 4027 ,(2003) , 10.1021/AC020736+