Transcriptional Analysis of Buffalo (Bubalus bubalis) Oocytes During In Vitro Maturation Using Bovine cDNA Microarray
作者： OM Kandil , N Ghanem , ASS Abdoon , M Hölker , C Phatsara
DOI: 10.1111/J.1439-0531.2008.01238.X
关键词: Gene expression 、 Oocyte 、 Microarray 、 In vitro maturation 、 Mitotic cell cycle 、 Biology 、 Molecular biology 、 Complementary DNA 、 Microarray analysis techniques 、 Regulation of gene expression
摘要: The need for improving in vitro production of buffalo embryos necessitates a better understanding the molecular mechanisms regulating early development including oocyte maturation. Here, we used bovine cDNA microarray platform to investigate mRNA abundance oocytes before and after For this, total six pools each contains 50 immature or matured were isolation subsequent synthesis. BlueChip (with approximately 2000 clones) was analyse gene expression profiles between oocytes. Statistical analysis data revealed 104 transcripts be differentially expressed two groups. Among these, transcription factors (ZFP91), M-phase mitotic cell cycle (MPHOSPH9), growth factor (BMP15) DNA binding (HMGN2) found up-regulated Similarly, enriched with genes involved cytoskeleton (ACTB), hydrogen ion transporting (ATP6V1C2) structural constituent ribosome (RPS27A). Quantitative real-time polymerase chain reaction validated profile some selected during array analysis. In conclusion, our knowledge, this is first large-scale study identify candidate abundant potential role
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