Identification of metaphase II-specific gene transcripts in porcine oocytes and their expression in early stage embryos.

摘要: Annealing control primer (ACP)-based GeneFishing polymerase chain reaction (PCR) was used to identify the genes that are specifically or prominently expressed in porcine oocytes at metaphase II (MII) and germinal vesicle (GV) stages. By using 60 ACPs, 13 differentially (DEGs) were identified. The cloned sequence tags (ESTs) showed similarity with known ESTs of other species GenBank. mRNA expression during oocyte maturation early embryonic development both pigs mice four these (namely transcription factor TZP, annexin A2, hypoxia-inducible protein 2, ATPase 6) further characterised by real-time quantitative reverse transcription–PCR. All markedly upregulated pig mouse MII compared GV-stage oocytes. levels decreased gradually cleavage. Thus, may play important roles and/or cleavage mammals. Although detailed functions remain be determined, their identification present study provides insights into meiotic fertilisation.