Effect of Scutellariae Radix Extract on the Proinflammatory Mediators in Raw 264.7 Cells Induced by LPS
作者： Young-Jong Lee , Hyo-Sang Han , Seok-Bin Yoon
DOI: 10.6116/KJH.2011.26.2.075
关键词: Immunology 、 Toxicity 、 Proinflammatory cytokine 、 Nitric oxide 、 RAW 264.7 Cells 、 Pharmacology 、 Cytokine 、 Viability assay 、 Chemistry 、 MTT assay 、 Radix
摘要: ABSTRACT Objectives：This study aims at examining the anti-inflammatory effects of Scutellariae Radix extract.Methods：Scutellariae was hot water extracted to make samples( SR) for experiment. Their were examined on increase cell viability in mouse macrophage Raw 264.7 cells, creation nitric oxide(NO) lipopolysaccharide(LPS)-induced a nd cytokines interleukin(IL)-1β and others.Results：The results experiment are as follows.1. The MTT assay carried out check cellular toxicity extract Radix. found no significant caused macrophages by 2. significantly restricted NO LPS-induced after 24-hour culture. 3. IL-6, IL-10, IL-12p40, IL-17, interferon-inducible protein(IP)-10, keratinocyte-derived chemokine(KC), vascular endothelial growth factor(VEGF) concentration 25 ㎍/mL or higher.Conclusion：The samples(SR) caus ed NO, IP-10, KC, VEGF higher, thus demonstrating effects.Key words：Scutellariae Radix, macrophage, cytokine, oxide.
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