Monocyte chemotactic protein-1 provokes mast cell aggregation and [3H]5HT release.

摘要: Monocyte chemotactic protein-1 (MCP-1) and MCP-3, the most active representative compounds of CC chemokine family, are proinflammatory cytokines that attract activate specific types leucocytes. We have used highly purified isolated rat peritoneal mast cells (RPMC) cultured for different lengths time with without MCP-1 (200, 100, 50 25 nM). Our data clearly show (200 nM) causes a marked release [3H]serotonin ([3H]5HT histamine, which reach peak at 40 min incubation (56.6 +/- 5.3 34.7 6 above control, respectively). In dose-response experiments, 50, 25, 12.5, 6.25 3.12 provoked dose-dependent [3H]5HT histamine from RPMC, was maximum 200 nM. After preparation histidine decarboxylase (HDC) probe, Northern blot analysis determined HDC mRNA. 4 hr, steady-state levels mRNA were induced in manner by (200-25 nM), compared to controls. However, failed prime RPMC when C48/80 (0.05 micrograms/ml) or anti-IgE used. contrast, murine interleukin-3 (IL-3) combination 100 greater than alone. Moreover, treated showed, under light microscopy (20x), clump formation, phenomenon absent controls (untreated cells). The electron microscope studies revealed treatment promoted binding demonstrated communication between cytoplasms adjacent cells. report describes additional biological activities MCP-1, suggesting first this human monocyte chemoattractant plays fundamental role serotonin cell aggregation